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51.
采用近红外光谱技术对落叶小乔木水冬瓜叶进行生药学鉴别研究,并建立快速真伪定性鉴别模型。采集42批次水冬瓜叶药材近红外原始光谱并对原始光谱进行预处理,运用主成分分析法(PCA)建立水冬瓜叶药材定性鉴别模型。同时分别采用6批次水冬瓜叶药材、水冬瓜根皮药材与水冬瓜树皮药材验证模型。结果表明:水冬瓜叶互生,呈阔心形,先端渐尖;叶表皮细胞体积膨大,附有非腺毛、表面有气孔;粉末中富含方晶,导管为螺纹导管,气孔为平轴式。建立的近红外快速定性鉴别模型可以快速、准确地鉴别出水冬瓜叶药材正品,预测准确率为100%,为水冬瓜叶药材质量评价提供了一种新技术与方法,可为今后水冬瓜叶的鉴定及深入研究提供鉴定依据。 相似文献
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AIM:To investigate the effects of siRNA targeting integrin-linked kinase (ILK) on the expression of glycogen synthase kinase 3β (GSK-3β) and β-catenin during epithelial-mesenchymal transition (EMT) in human kidney proximal tubular epithelial cell line HKC induced by high glucose. METHODS:HKC cells were divided into 4 groups:normal glucose (NG) group, high glucose (HG) group, HG+HK (a vector containing the non-specific siRNA designed as negative control) group and HG+ILK siRNA group. The inverted fluorescence microscope was used to examine the expression of green fluorescent protein (GFP). The expression of ILK at mRNA and protein levels was detected by RT-PCR and Western blotting. The expression of p-GSK-3β and β-catenin was observed by immunocytochemical staining. The protein expression of total GSK-3β, p-GSK-3β, nuclear β-catenin, total β-catenin, E-cadherin and α-smooth muscle actin (α-SMA) was measured by Western blotting. RESULTS:GFP was observed in HKC cells, indicating that the transfection was successful. Both the protein and mRNA of ILK were down-regulated in HG+ILK siRNA group compared with HG group and HG+HK group, but still higher than those in NG group. Silencing of ILK down-regulated the expression of p-GSK-3β and nuclear β-catenin. No difference of total GSK-3β or total β-catenin was observed among the 4 groups. CONCLUSION:These data support a functional role of ILK, GSK-3β and β-catenin in tubular EMT induced by high glucose. ILK may promote tubular EMT by regulating the activity of GSK-3β and β-catenin, the downstream effectors of the Wnt/β-catenin pathway. 相似文献
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AIM:To study the effect of small interfering RNA (siRNA) on the expression of beta 2-microglo-bulin (β2M) in pre-differentiated bone marrow mesenchymal stem cells (BMSCs). METHODS:The β2M siRNA was transfected into the pre-differentiated BMSCs with Lipofectamine 2000. BMSCs were divided into transfection group, blank control group and negative control group. The expression of β2M at mRNA and protein levels was determined by real-time qPCR, Western blotting and laser confocal microscopy. The productions of aggrecan and type II collagen in pre-differentiated BMSCs were determined by toluidine blue staining and type Ⅱ collagen immunofluorescence. RESULTS:The results of real-time qPCR, Western blotting and laser confocal microscopy showed that siRNA successfully inhibited the expression of β2M at mRNA and protein levels in the pre-differentiated BMSCs. The results of toluidine blue and type Ⅱ collagen immunofluorescence staining showed that siRNA does not affect the productions of aggrecan and type Ⅱ collagen in the pre-differentiated BMSCs. CONCLUSION:siRNA targeting β2M reduces the expression of β2M in the pre-differentiated BMSCs and does not affect the chondrocyte characteristics of pre-differentiated BMSCs. 相似文献
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为了探究黄瓜苗期生长发育规律和育苗方式,以华南型黄瓜‘沪杂7号’和荷短型黄瓜‘春秋王2号’为试材,用卡尺对植株高度、茎粗度、子叶大小、叶片大小进行测量,用天平称量地上部、根鲜质量,电子秤称量产量,利用LI-6400型光合仪对气体交换参数净光合速率(Pn)、气孔导度(Gs)、胞间CO2浓度(Ci)与蒸腾速率(Tr)进行测量。结果表明,黄瓜植株高度、茎粗度增长均似线形,育苗后期生长速度更快,叶片长度、宽度增长速度前期较快,后期略慢;定植前大苗光合作用明显好于小苗,地上部、根鲜质量均显著高于小苗;华南型、荷短型黄瓜大苗定植,较小苗定植采收期均提前10 d以上,同时总产量明显增加。大苗定植对黄瓜提前上市及增加收入有明显效果。 相似文献
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采用AutoCAD工作软件设计了一款链式小型林业原条输送装备,并对其中的关键机构的设计与计算过程进行了翔实表述。按该设计方案制造出的链式原条输送机采用链条传动,与带传动相比,无弹性滑动和打滑现象,平均传动比准确,工作可靠,效率高;该机械传递功率大,过载能力强,相同工况下的传动尺寸小;所需紧张力小,作用于轴上压力小。同时,采用特殊滚筒式输送平台,不仅对原条起到了导向作用,而且可减少原条在行进过程中的左右方向上的移动,有效地降低了能耗,提高了生产效率。该设备能在高温、潮湿、多尘、有污染的恶劣环境下工作,适合于林区环境工作。 相似文献
56.
降雨类型和水土保持对黄土区小流域水土流失的影响 总被引:15,自引:0,他引:15
对黄土区桥子东沟流域的143场次洪水事件水文泥沙数据进行统计分析,并利用K均值分类法划分降雨类型,比较不同降雨类型条件下流域水土流失特征,探讨水土保持治理对不同降雨类型下流域产流输沙的影响。结果表明,次降雨量和最大30 min雨强是影响流域产流输沙的主要降雨特征。降雨事件划分为4种类型:Ⅰ型(小雨量、小雨强)、Ⅱ型(大雨量、大雨强)、Ⅲ型(大雨量、小雨强)和Ⅳ型(小雨量、大雨强)。4种降雨类型下次洪水事件的产流能力和洪峰流量由大到小依次为Ⅱ型、Ⅳ型、Ⅲ型和Ⅰ型。频次最少的Ⅱ型降雨次洪水事件的输沙量最多,Ⅰ型、Ⅲ型和Ⅳ型降雨条件下次洪水事件输沙量的差异不显著,输沙量均较少。水土保持综合治理显著减少了流域径流量和输沙量,其中Ⅱ型降雨次洪水事件减水量和减沙量最多,Ⅰ型最少。 相似文献
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Jeffrey C. Mangel Joanna Alfaro-Shigueto Koen Van Waerebeek 《Biological conservation》2010,143(1):136-143
We detail the first direct, at sea monitoring of small cetacean interactions with Peruvian artisanal drift gillnet and longline fisheries. A total of 253 small cetaceans were captured during 66 monitored fishing trips (Gillnet: 46 trips; Longline: 20 trips) from the port of Salaverry, northern Peru (8o14′S, 78o59′W) from March 2005 to July 2007. The most commonly captured species were common dolphins (Delphinus spp.) (47%), dusky dolphins (Lagenorhynchus obscurus) (29%), common bottlenose dolphins (Tursiops truncatus) (13%) and Burmeister’s porpoises (Phocoena spinipinnis) (6%). An estimated 95% of common dolphin bycatch was of long-beaked common dolphins (Delphinus capensis). Overall bycatch per unit effort for gillnet vessels (mean ± sd) was estimated to be 0.65 ± 0.41 animals.set−1 (range 0.05-1.50) and overall catch (bycatch and harpoon) was 4.96 ± 3.33 animals.trip−1 (range 0.33-13.33). Based upon total fishing effort for Salaverry we estimated the total annual average small cetacean bycatch by gillnet vessels as 2412 animals.year−1 (95% CI 1092-4303) for 2002-2007. This work indicates that, in at least one Peruvian port, bycatch and harpooning of small cetaceans persist at high levels and on a regular basis, particularly in driftnet vessels, despite the existence since the mid-1990s of national legislation banning the capture of marine mammals and commerce in their products. It is concluded that the coast of Peru is likely still one of the world’s principal areas for concern regarding high small cetacean bycatch and there is clearly an urgent need to increase the geographic scope of observer effort to elucidate the full magnitude of this issue. 相似文献
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